Construction of recombinant plasmids containing rat muscle actin and myosin light chain DNA sequences.
نویسندگان
چکیده
The construction and partial characterization of recombinant bacterial plasmids carrying DNA sequences that hybridize with rat skeletal muscle actin and a myosin light chain mRNA is described. DNA of one clone hybridizes specifically with the muscle-specific alpha-actin mRNA. Three plasmid clones contain DNA inserts that hybridize with muscle as well as with nonmuscle actin mRNA. A fifth plasmid contains sequences complementary to mRNA coding for myosin light chain 2. DNA of this plasmid hybridizes specifically with RNA extracted from muscle and differentiated muscle cultures but not with RNA extracted from proliferating mononucleated myogenic cells.
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عنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 77 2 شماره
صفحات -
تاریخ انتشار 1980